Abstract : The glucosidase enzymes are responsible for carbohydrate metabolism and increase free glucose in blood, which is one of the major causes of diabetes. This study evaluates alpha-amylase inhibitory effects (in vitro) of some popularly used Ayurvedic, Unani, and Homeopathic drugs taking an Allopathic standard, acarbose (sugatrol). Spectrophotometric method was used for this purpose. Results suggest that the Homeopathic drug Syzygium jambolinum at all concentration strongly inhibited glucosidase activity in comparison to the other drugs of the same a different treatment systems. The Ayurvedic drug, Jambadharists also inhibited alpha-amylase activity significantly (p <0.01 and p <0.001) in comparison to the control group. The standard Alopathic drug, Sugatrol at 200 µg/mL inhibited alpha-amylase by 78.31%. In conclusion, S. jambolinum and Jambadharists have a glycosidase inhibitory effect.